Project information
Czech Infrastructure for Integrative Structural Biology
(CIISB)
- Project Identification
- LM2018127
- Project Period
- 1/2020 - 12/2022
- Investor / Pogramme / Project type
-
Ministry of Education, Youth and Sports of the CR
- Large Infrastructures for Research, Development and Innovation
- MU Faculty or unit
-
Central European Institute of Technology
- prof. RNDr. Vladimír Sklenář, DrSc.
- Cooperating Organization
-
Institute of Biotechnology CAS
The Czech Infrastructure for Integrative Structural Biology (CIISB) is a distributed infrastructure of the core facilities and central laboratories of CEITEC (Central European Institute of Technology, Brno) and BIOCEV (Biotechnology and Biomedicine Centre, Vestec) providing expertise and access to technologies used for integrative approaches to structural analysis of biologically important cellular components and macromolecules - proteins, nucleic acids, and their complexes. CIISB, equipped with the cutting-edge technologies, will offer access to the equipment and expertise in the following fields: high-field NMR spectroscopy; high throughput crystallization of biological macromolecules; X-ray diffraction techniques; Bio-SAXS measurements; AFM imaging; characterization of proteins, nucleic acids and complexes by biophysical methods including microcalorimetry, dynamic light scattering, and surface plasmon resonance; high-end cryo-electron microscopy and tomography allowing studies of cellular structures, organelles, and biomacromolecular complexes by 3D imaging; protein expression in eukaryotic cells; high-end mass spectrometry characterization of biomacromolecules; determination of protein post-translational modifications; MS-based peptide mapping/sequencing; high throughput sequencing and proteomic services. CIISB infrastructure aims to provide a high quality open access services in provision of methods and techniques on a level of national and trans-national importance. CIISB is a part of the ESFRI project INSTRUCT-ERIC.
Sustainable Development Goals
Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.
Publications
Total number of publications: 328
2021
-
Oxygen Is an Ambivalent Factor for the Differentiation of Human Pluripotent Stem Cells in Cardiac 2D Monolayer and 3D Cardiac Spheroids
International Journal of Molecular Sciences, year: 2021, volume: 22, edition: 2, DOI
-
p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice
Communications Biology, year: 2021, volume: 4, edition: 1, DOI
-
Phosphorylation within Intrinsic Disordered Region Discriminates Histone Variant macroH2A1 Splicing Isoforms-macroH2A1.1 and macroH2A1.2
Biology, year: 2021, volume: 10, edition: 7, DOI
-
Phosphorylation-induced changes in the PDZ domain of Dishevelled 3
Scientific Reports, year: 2021, volume: 11, edition: 1, DOI
-
Photon-upconversion nanoparticles as a background-free label in lateral flow immunoassays
Year: 2021, type: Conference abstract
-
PLK1 regulates the PrimPol damage tolerance pathway during the cell cycle
Science Advances, year: 2021, volume: 7, edition: 49, DOI
-
PLL-like lectin family from entomopathogenic bacterium Photorhabdus laumondii
Year: 2021, type: Appeared in Conference without Proceedings
-
Polymer pencil leads as a porous nanocomposite graphite material for electrochemical applications: The impact of chemical and thermal treatments
Electrochemistry Communications, year: 2021, volume: 126, edition: May, DOI
-
Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
Jove-Journal of Visualized Experiments, year: 2021, volume: NOV, edition: 177, DOI
-
Proteotypová analýza renálního karcinomu ve vztahu k prognóze a terapeutické odpovědi
Year: 2021, type: Appeared in Conference without Proceedings