Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling

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Authors	MÉNOVÁ Petra CAHOVÁ Hana PLUCNARA Medard HAVRAN Luděk FOJTA Miroslav HOCEK Michal
Year of publication	2013
Type	Article in Periodical
Magazine / Source	Chemical communications
MU Faculty or unit	Central European Institute of Technology
Citation
Web	http://www.rsc.org/chemcomm
Doi	http://dx.doi.org/10.1039/C3CC41438H
Field	Biochemistry
Keywords	DNA polymerase; DNA labelling; oligonucleotides
Description	Enzymatic construction of single-nucleobase redox-labelled oligonucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magnetoseparation and the second PEX with a full-length template.
Related projects:	Centrum nových přístupů k bioanalýze a molekulární diagnostice