RNA-dependent disassembly of nuclear bodies

This publication doesn't include Faculty of Arts. It includes Faculty of Informatics. Official publication website can be found on muni.cz.

Authors

MUSINOVA Yana R. LISITSYNA Olga M. SOROKIN Dmitry ARIFULIN Eugene A. SMIRNOVA Tatiana A. ZINOVKIN Roman A. POTASHNIKOVA Daria M. VASSETZKY Yegor S. SHEVAL Eugene V.

Year of publication 2016
Type Article in Periodical
Magazine / Source Journal of Cell Science
MU Faculty or unit

Faculty of Informatics

Citation
Doi http://dx.doi.org/10.1242/jcs.189142
Field Genetics and molecular biology
Keywords Nuclear bodies; Nucleolus; Interphase prenucleolar body; pre-rRNA; B23; NPM1; Self-organization
Description Nuclear bodies are membraneless organelles that play important roles in genome functioning. A specific type of nuclear bodies known as interphase prenucleolar bodies (iPNBs) are formed in the nucleoplasm after hypotonic stress from partially disassembled nucleoli. iPNBs are then disassembled, and the nucleoli are reformed simultaneously. Here, we show that diffusion of B23 molecules (also known as nucleophosmin, NPM1) from iPNBs, but not fusion of iPNBs with the nucleoli, contributes to the transfer of B23 from iPNBs to the nucleoli. Maturation of pre-ribosomal RNAs (rRNAs) and the subsequent outflow of mature rRNAs from iPNBs led to the disassembly of iPNBs. We found that B23 transfer was dependent on the synthesis of pre-rRNA molecules in nucleoli; these pre-rRNA molecules interacted with B23 and led to its accumulation within nucleoli. The transfer of B23 between iPNBs and nucleoli was accomplished through a nucleoplasmic pool of B23, and increased nucleoplasmic B23 content retarded disassembly, whereas B23 depletion accelerated disassembly. Our results suggest that iPNB disassembly and nucleolus assembly might be coupled through RNA-dependent exchange of nucleolar proteins, creating a highly dynamic system with long-distance correlations between spatially distinct processes.
Related projects: