Paramagnetic nanoparticles in stem cell therapy

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Year of publication 2016
Type Conference abstract
MU Faculty or unit

Faculty of Informatics

Description In recent years metal oxide nanoparticles have become a useful tool in biomedicine. They are used as contrast agents for magnetic resonance imaging, carriers in drug delivery, compounds for hyperthermia cancer treatment or targets for cell tracking and guiding mechanisms. Even though recent advances bring stem cell therapy closer to clinical application, several issues must be addressed. The success of the healing process depends on precision targeting into the site of the injury. Nanoparticles provide a mechanism that effectively guides cells to the site of the action. Recently gamma–Fe2O3 and Fe3O4 were synthetized by microwave plasma torch. We studied the interaction and endocytosis of the nanoparticles with various cell cultures in order to optimize the process for future clinical application. We focused on cells with different stem properties, including human neonatal fibroblasts, human umbilical vein endothelial cells, adult vein endothelial cells and pluripotent stem cells. We confirmed the location of the nanoparticles by cell histology as well as electron microscopy. We assessed the effects of nanoparticles on cell viability, proliferation and metabolism by Annexin V and MTT assay. We observed differences in the accumulation of nanoparticles in cell cytoplasm and cell survival. We were able to achieve a level of saturation of NPs that produced cells capable of magnetic separation with minimal toxic effects. These properties favor the use of gamma–Fe2O3 and Fe3O4 synthetized by microwave plasma torch in stem cell therapy.
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