TWISTED DWARF1 Mediates the Action of Auxin Transport Inhibitors on Actin Cytoskeleton Dynamics

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Authors

ZHU Jinsheng BAILLY Aurelie ZWIEWKA Marta SOVERO Valpuri DI DONATO Martin GE Pei OEHRI Jacqueline ARYAL Bibek HAO Pengchao LINNERT Miriam BURGARDT Noelia Ines LUCKE Christian WEIWAD Matthias MICHEL Max WEIERGRABER Oliver H. POLLMANN Stephan AZZARELLO Elisa MANCUSO Stefano FERRO Noel FUKAO Yoichiro HOFFMANN Celine WEDLICH-SOLDNER Roland FRIML Jiri THOMAS Clement GEISLER Markus

Year of publication 2016
Type Article in Periodical
Magazine / Source Plant Cell
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://www.plantcell.org/content/28/4/930
Doi http://dx.doi.org/10.1105/tpc.15.00726
Field Biochemistry
Keywords ACID-BINDING PROTEIN; ARABIDOPSIS-THALIANA; PLASMA-MEMBRANE; PLANT DEVELOPMENT; ABC TRANSPORTERS; P-GLYCOPROTEINS; CORTICAL ARRAY; INTRACELLULAR TRAFFICKING; FUNCTIONALLY INTERACTS; EFFLUX TRANSPORTER
Description Plant growth and architecture is regulated by the polar distribution of the hormone auxin. Polarity and flexibility of this process is provided by constant cycling of auxin transporter vesicles along actin filaments, coordinated by a positive auxin-actin feedback loop. Both polar auxin transport and vesicle cycling are inhibited by synthetic auxin transport inhibitors, such as 1-N-naphthylphthalamic acid (NPA), counteracting the effect of auxin; however, underlying targets and mechanisms are unclear. Using NMR, we map the NPA binding surface on the Arabidopsis thaliana ABCB chaperone TWISTED DWARF1 (TWD1). We identify ACTIN7 as a relevant, although likely indirect, TWD1 interactor, and show TWD1-dependent regulation of actin filament organization and dynamics and that TWD1 is required for NPA-mediated actin cytoskeleton remodeling. The TWD1-ACTIN7 axis controls plasma membrane presence of efflux transporters, and as a consequence act7 and twd1 share developmental and physiological phenotypes indicative of defects in auxin transport. These can be phenocopied by NPA treatment or by chemical actin (de) stabilization. We provide evidence that TWD1 determines downstreamlocations of auxin efflux transporters by adjusting actin filament debundling and dynamizing processes and mediating NPA action on the latter. This function appears to be evolutionary conserved since TWD1 expression in budding yeast alters actin polarization and cell polarity and provides NPA sensitivity.
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