Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide

Warning

This publication doesn't include Faculty of Arts. It includes Central European Institute of Technology. Official publication website can be found on muni.cz.

Authors	SUN Xiaolong ODYNIEC Maria L. SEDGWICK Adam C. LACINA Karel XU Suying QIANG Taotao BULL Steven D. MARKEN Frank JAMES Tony D.
Year of publication	2017
Type	Article in Periodical
Magazine / Source	Organic Chemistry Frontiers
MU Faculty or unit	Central European Institute of Technology
Citation
Web	http://pubs.rsc.org/en/content/articlepdf/2017/qo/c6qo00448b
Doi	http://dx.doi.org/10.1039/c6qo00448b
Keywords	FLUORESCENT-PROBES; LIVING CELLS; BORONIC ACID; IN-VIVO; INFLAMMATION; REPORTER; MODEL
Description	Based on the complexation of phenylboronic acid (PBA) with Alizarin Red S (ARS), we developed a new chemosensor for the detection of hydrogen peroxide (H2O2) in aqueous media. This platform has demonstrated its ability to detect H2O2 via colorimetric, fluorometric, and electrochemical measurements. The experimental observations reveal that the system displays a red-shifted visible colour change, on–off fluorescence response indicating release of indicator (ARS) and turn-on electrochemical signal indicating generation of phenol, after reaction with H2O2. With this work we have demonstrated that our reaction-based indicator displacement assay (RIA) systems, can be employed as an assay for H2O2 and hydrogen peroxide-related species for environmental and physiological detection.
Related projects:	CEITEC 2020