Laser-Induced Breakdown Spectroscopy as a Novel Readout Method for Nanoparticle-Based Immunoassays

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Authors

MODLITBOVÁ Pavlína FARKA Zdeněk PASTUCHA Matěj POŘÍZKA Pavel NOVOTNÝ Karel SKLÁDAL Petr KAISER Jozef

Year of publication 2019
Type Conference abstract
MU Faculty or unit

Central European Institute of Technology

Citation
Description Laser-Induced Breakdown Spectroscopy (LIBS) was examined as a novel method to provide the readout of microtiter plate immunoassays involving nanoparticles (NP). The so-called Tag-LIBS, i.e. a sensitive detection of specific biomarkers, was applied to detect the NP labels using the nanosecond ablation sampling. The NP labels were examined from the bottom of the standard 96-well microtiter plate. Thanks to the well-known flexibility in the LIBS instrumentation, the plasma emission collection as well as the focusing optics can be rebuilt into a collinear arrangement. The presented experiments proved the suitable detection of silver NPs from the microtiter plate’s bottom without any negative effects on the NPs’ qualitative and quantitative analysis. Subsequently, a sandwich immunoassay tha tdetects human serum albumin was successfully developed, using streptavidin-coated AgNP labels. The assay provided a detection limit of 10 ng/mL, which is comparable with the fluorescence-based readout. The main advantage of LIBS readout is that it enables a detection of any type of NP labels independent on their fluorescence or catalytic properties. Owing to the immediate signal response, a relatively simple instrumentation enabling the automation, and the capability of multi-elemental analyses, LIBS technique offers a promising and fast alternative to other readout techniques allowing a multiplex detection of various biomarkers.
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