Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethylsulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability

Authors

KRATOCHVÍLOVÁ I GOLAN M POMEISL K RICHTER J SEDLÁKOVÁ S ŠEBERA J MIČOVÁ J FALK Martin FALKOVÁ I ŘEHA D ELLIOTT K W VARGA K FOLLETT S E ŠIMEK D

Year of publication 2017
Type Article in Periodical
Magazine / Source RSC Advances
Citation
Web http://dx.doi.org/10.1039/c6ra25095e
Doi http://dx.doi.org/10.1039/c6ra25095e
Description In this work the physico-chemical properties of selected cryoprotectants (antifreeze protein TrxA-AFP752, trehalose and dimethyl sulfoxide) were correlated with their impact on the constitution of ice and influence on frozen/thawed cell viability. The freezing processes and states of investigated materials solutions were described and explained from a fundamental point of view using ab initio modelling (molecular dynamics, DFT), Raman spectroscopy, differential scanning calorimetry and X-ray diffraction. For the first time, in this work we correlated the microscopic view (modelling) with the description of the frozen solution states and put these results in the context of human skin fibroblast viability after freezing and thawing. DMSO and AFP had different impacts on their solution's freezing process but in both cases the ice crystallinity size was considerably reduced. DMSO and AFP treatment in different ways improved the viability of frozen/thawed cells.
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