Gene expression and metabolic activity of Streptococcus mutans during exposure to dietary carbohydrates glucose, sucrose, lactose, and xylitol

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Authors

ŽAJDLÍKOVÁ Veronika FARKOVÁ Veronika KUČERA Jiří DADÁKOVÁ Kateřina ZAPLETALOVÁ Martina PAŠKOVÁ Kateřina ŘEMÍNEK Roman GLATZ Zdeněk IZAKOVIČOVÁ HOLLÁ Lydie RŮŽIČKA Filip LOCHMAN Jan BOŘILOVÁ LINHARTOVÁ Petra

Year of publication 2023
Type Article in Periodical
Magazine / Source Molecular Oral Microbiology
MU Faculty or unit

Faculty of Science

Citation
Web https://doi.org/10.1111/omi.12428
Doi http://dx.doi.org/10.1111/omi.12428
Keywords cariogenic carbohydrates; cell wall; metabolome; RNA-Seq; Streptococcus mutans; transcriptome
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Description Recent RNA sequencing studies have given us a deeper insight into the cariogenic impact of carbohydrate sources in the bacterium Streptococcus mutans, the principal microbial agent in dental caries etiopathogenesis. The process of dental caries development is facilitated by the ability of this bacterium to ferment some carbohydrates into organic acids contributing to a pH decrease in the oral cavity and the demineralization of the hard tissues of the tooth. Furthermore, in dental caries progression, biofilm formation, which starts and ends with free planktonic cells, plays an important role and has several unique properties called virulence factors. The most cariogenic carbohydrate is sucrose, an easily metabolizable source of energy that induces the acidification and synthesis of glucans, forming typical bacterial cell clumps. We used multifaceted methodological approaches to compare the transcriptomic and metabolomic profiles of S. mutans growing in planktonic culture on preferred and nonpreferred carbohydrates and in fasting conditions. Streptococcus mutans in a planktonic culture with lactose produced the same pH drop as glucose and sucrose. By contrast, xylitol and lactose showed high effectiveness in regulating intracellular polysaccharide metabolism, cell wall structure, and overall virulence involved in the initial phase of biofilm formation and structure but with an opposite pattern compared with sucrose and glucose. Our results confirmed the recent findings that xylitol and lactose play a vital role in biofilm structure. However, they do not reduce its formation, which is related to the creation of a cariogenic environment.
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