Novel multipurpose matrix for a high resolution MALDI MS and multimodal imaging
| Authors | |
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| Year of publication | 2025 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
| Citation | |
| Description | The staining with basic fuchsin streamlines sample preparation, eliminating the need for matrix spraying or sublimation, and enables true multimodal analysis on a single tissue section thanks to its compatibility with all employed imaging modalities. Innate fluorescence of basic fuchsin ensuresthat no additional sample treatment or addition of other fluorophores is needed for recording fluorescence images. The technique is demonstrated on mapping of mouse brain: MALDI MSI modality visualizes spatial distribution of lipids and fluorescence combined with histology clearly defines the different areas and tissue types in the brain section. Furthermore, MALDI MS with basic fuchsin is usable with laser postionization, i.e. MALDI-2, to increase sensitivity and spatial resolution to 5 micrometers. The high-quality of recorded images is comparable to the MALDI/MALDI-2 MSI with classical matrices. The developed multimodal workflow facilitates high-resolution molecular imaging and offers tools for holistic characterization of biological tissues. |
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