Determination of tick positivity for pathogenic Borrelia burgdorferi by using PCR-RFLP method
| Authors | |
|---|---|
| Year of publication | 2004 |
| Type | Article in Periodical |
| Magazine / Source | Scripta medica |
| MU Faculty or unit | |
| Citation | |
| Field | Immunology |
| Keywords | tick; Borrelia burgdorferi;Lyme disease; DFM; PCR; PCR-RFLP |
| Description | The aim of the study was the monitoring of the presence of Borrelia burgdorferi in ticks Ixodes ricinus in the Pisárky park, Brno in 1996-2001. Ticks were analysed by DFM and next by the PCR method. The samples with a greater mass of borrelia DNA were analysed by the PCR-RFLP method and transfered into BSK-H medium and cultivated. |
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