The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1

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Authors	HAVLIŠ Jan OELSCHLAEGEL Tobias SCHWICKART Martin MATOS Joao BOGDANOVA Aliona CAMASSES Alain SHEVCHENKO Andrej ZACHARIAE Wolfgang
Year of publication	2005
Type	Article in Periodical
Magazine / Source	Cell
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	anaphase-promoting complex; chromosome segregation; fission yeast; centromeric cohesion; spore formation; protein family; kinase cdc5; cleavage; mitosis; proteolysis
Description	Cohesion established between sister chromatids during pre-meiotic DNA replication mediates two rounds of chromosome segregation. The first division is preceded by an extended prophase wherein homologous chromosomes undergo recombination. The persistence of cohesion during prophase is essential for recombination and both meiotic divisions. Here we show that Mnd2, a subunit of the anaphase-promoting complex (APC/C) from budding yeast, is essential to prevent premature destruction of cohesion in meiosis. During S- and prophase, Mnd2 prevents activation of the APC/C by a melosis-specific activator called Ama1. In cells lacking Mnd2 the APC/C-Ama1 enzyme triggers degradation of Pds1, which causes premature sister chromatid separation due to unrestrained separase activity. In vitro, Mnd2 inhibits ubiquitination of Pds1 by APC/C-Ama1 but not by other APC/C holo-enzymes. We conclude that chromosome segregation in meiosis depends on the selective inhibition of a meiosis-specific form of the APC/C.