Energy coupling to nitrate uptake into the denitrifying cells of Paracoccus denitrificans

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Authors	KUČERA Igor
Year of publication	2005
Type	Article in Periodical
Magazine / Source	Biochimica et Biophysica Acta Bioenergetics
MU Faculty or unit	Faculty of Science
Citation
web	http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T1S-4GTVW4N-1&_user=835458&_handle=V-WA-A-W-AU-MsSAYWW-UUA-U-AABWWUCCCU-AAWEDYZBCU-VAUEDAYAC-AU-U&_fmt=full&_coverDate=09%2F05%2F2005&_rdoc=3&_orig=browse&_srch=%23toc%234898%232005%23982909997%2
Field	Biochemistry
Keywords	nitrate transport denitrification bacteria energization ionophores
Description	This study deals with the effects of the agents that dissipate the individual components of the proton motive force (short-chain fatty acids, nigericin, and valinomycin) upon the methyl viologen-coupled nitrate reductase activity in intact cells. The data indicate that nitrate transport relies heavily on the pH gradient at neutral pH. A potential dependent step may be of significance in the transporter cycle under alkaline pH. Experiments with sodium-depleted media provided no hints for Na+ as a possible H+ substitute.
Related projects:	Proteins in metabolism and interaction of organisms with the environment