A new, sensitive method for enzyme kinetic studies of scarce glucosides.

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Authors	MAZURA Pavel FOHLEROVÁ Radka BRZOBOHATÝ Břetislav KIRAN Nagavalli Subbanna JANDA Lubomír
Year of publication	2006
Type	Article in Periodical
Magazine / Source	JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	Amplex UltraRed reagent; beta-glucosidase; glucose assay; glucose-oxidase / peroxidase coupled reaction; phytohormone conjugation; zeatin-O-glucoside
Description	We have developed a rapid, accurate and sensitive assay for glucose that enabled us to measure the kinetic parameters for a cytokinin-O-glucoside-specific beta-glucosidase towards its natural substrate. Both the beta-glucosidase reaction and the detection reaction occur simultaneously in solution. Measurement is continuous and the reaction is measured nine times during three seconds.
Related projects:	Molecular basis of cell and tissue regulations Regulation of morphogenesis of plant cells and organs