Identification of antifungal proteins in the barley and malt caryopsis by mass spectrometry

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Authors	HÉGROVÁ Blanka MACUCHOVÁ Simona PREISLER Jan HAVEL Josef
Year of publication	2008
Type	Article in Proceedings
Conference	Vitamins 2008 Nutrition and Diagnostics
MU Faculty or unit	Faculty of Science
Citation
Field	Analytic chemistry
Keywords	low molecular weight proteins; barley; malt; SDS-PAGE; enzymatic digestion; MALDI-TOF mass spectrometry
Description	Recently, great attention is paid to determination of various metabolites after pathogen-caryopsis interaction. Some of antifungal proteins, e.g. thionins, might be of effect on barley quality and its subsequent food products - gushing or premature yeast flocculation. The changes proceeding during pathogen-caryopsis interaction were monitored in 20 samples of barley and its corresponding malt by the combination of Sodium Dodecyl Sulphate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Matrix-Assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry (MALDI-TOF MS).
Related projects:	Study of the chemical changes of barley caryopses after an attack by pathogens to monitor and improve the quality of barley and its derived food products