Selective isolation of DNA from food matrices

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Authors	ŠPANOVÁ Alena TVRDÍKOVÁ Jana HORÁK Daniel RITTICH Bohuslav
Year of publication	2010
Type	Article in Proceedings
Conference	30th International Symposium on the Separation of Proteins, Peptides and Polynucleotides
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	magnetic particles; streptavidin; Lactobacillus DNA separation; PCR
Description	Research has been focused on the development of innovative magnetic micrometer-sized microspheres for specific applications in DNA analysis in food matrices and other real samples. Selective isolation of high-quality DNA can be performed using suitable functionalised magnetic microspheres. Magnetic P(HEMA-co-GMA) microspheres functionalised with streptavidin were used for immobilisation of biotinylated DNA probes (primers, PCR products) and for selective DNA isolation of Lactobacillus paracasei using DNA/DNA hybridisation. The procedure proposed was applied for target isolation of DNA from probiotic bacteria in complex samples (probiotic food supplements). Isolated DNA was used in genus- and species-specific PCRs.
Related projects:	Molecular basis of cell and tissue regulations New methods for the characterization and identification of probiotic bacterial strains suitable for functional foods