Validation of Minim typing for fast and accurate discrimination of extended-spectrum, beta-lactamase-producing Klebsiella pneumoniae isolates in tertiary care hospital

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Authors

BRHELOVÁ Eva KOCMANOVA Iva RÁČIL Zdeněk HANSLIANOVA Marketa ANTONOVA Mariya MAYER Jiří LENGEROVÁ Martina

Year of publication 2016
Type Article in Periodical
Magazine / Source Diagnostic Microbiology and Infectious Disease
MU Faculty or unit

Faculty of Medicine

Citation
Web http://ac.els-cdn.com/S0732889316300505/1-s2.0-S0732889316300505-main.pdf?_tid=1e0a88a6-84be-11e6-a6d2-00000aab0f6c&acdnat=1474986456_687cb42e2b0877c46e8213dd759647c5
Doi http://dx.doi.org/10.1016/j.diagmicrobio.2016.03.010
Field Microbiology, virology
Keywords Klebsiella pneumoniae; ESBL; High-resolution melt analysis; Multi-locus sequence typing; Minim typing
Attached files
Description Minim typing is derived from the multi-locus sequence typing (MIST). It targets the same genes, but sequencing is replaced by high resolution melt analysis. Typing can be performed by analysing six loci (6MelT), four loci (4MelT) or using data from four loci plus sequencing the tonB gene (HybridMelT). The aim of this study was to evaluate Minim typing to discriminate extended-spectrum beta-lactamase producing Klebsiella pneumoniae (ESBL-KLPN) isolates at our hospital. In total, 380 isolates were analyzed. The obtained alleles were assigned according to both the 6MelT and 4MelT typing scheme. In 97 isolates, the tonB gene was sequenced to enable HybridMelT typing. We found that the presented method is suitable to quickly monitor isolates of ESBL-KLPN; results are obtained in less than 2 hours and at a lower cost than MLST. We identified a local ESBL-KLPN outbreak and a comparison of colonizing and invasive isolates revealed a long term colonization of patients with the same strain.
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