Validation of Minim typing for fast and accurate discrimination of extended-spectrum, beta-lactamase-producing Klebsiella pneumoniae isolates in tertiary care hospital

Varování

Publikace nespadá pod Filozofickou fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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BRHELOVÁ Eva KOCMANOVA Iva RÁČIL Zdeněk HANSLIANOVA Marketa ANTONOVA Mariya MAYER Jiří LENGEROVÁ Martina

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj Diagnostic Microbiology and Infectious Disease
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www http://ac.els-cdn.com/S0732889316300505/1-s2.0-S0732889316300505-main.pdf?_tid=1e0a88a6-84be-11e6-a6d2-00000aab0f6c&acdnat=1474986456_687cb42e2b0877c46e8213dd759647c5
Doi http://dx.doi.org/10.1016/j.diagmicrobio.2016.03.010
Obor Mikrobiologie, virologie
Klíčová slova Klebsiella pneumoniae; ESBL; High-resolution melt analysis; Multi-locus sequence typing; Minim typing
Přiložené soubory
Popis Minim typing is derived from the multi-locus sequence typing (MIST). It targets the same genes, but sequencing is replaced by high resolution melt analysis. Typing can be performed by analysing six loci (6MelT), four loci (4MelT) or using data from four loci plus sequencing the tonB gene (HybridMelT). The aim of this study was to evaluate Minim typing to discriminate extended-spectrum beta-lactamase producing Klebsiella pneumoniae (ESBL-KLPN) isolates at our hospital. In total, 380 isolates were analyzed. The obtained alleles were assigned according to both the 6MelT and 4MelT typing scheme. In 97 isolates, the tonB gene was sequenced to enable HybridMelT typing. We found that the presented method is suitable to quickly monitor isolates of ESBL-KLPN; results are obtained in less than 2 hours and at a lower cost than MLST. We identified a local ESBL-KLPN outbreak and a comparison of colonizing and invasive isolates revealed a long term colonization of patients with the same strain.
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