Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94

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Authors

PRUDNIKOVA Tanyana CHALOUPKOVÁ Radka SATO Yukari NAGATA Yuji DEGTJARIK O. KUTÝ Michal REZACOVA P. DAMBORSKÝ Jiří KUTA-SMATANOVÁ Smatanova I.

Year of publication 2011
Type Article in Periodical
Magazine / Source Crystal Growth & Design
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1021/cg1013363
Field Biochemistry
Keywords Crystallization; X-ray;Haloalkane dehalogenases
Description The DbeA1 variant of a novel haloalkane dehalogenaseDbeA (EC 3.8.1.5) from Bradyrhizobium elkani USDA94 was constructed to study the structure-function relationships between DbjA and DbeA enzymes. A DbeA1 variant carries a unique fragment of nine amino acids transplanted from the sequentially closely related enzyme DbjA from Bradyrhizobium japonicum USDA110 to DbeA. Here we report development of the crystallization protocol for DbeA1 and soaking experiments with the ligands 1-fluoropentane and 1,3-dichloropropane.
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