Project information
Czech Infrastructure for Integrative Structural Biology
(CIISB)
- Project Identification
- LM2018127
- Project Period
- 1/2020 - 12/2022
- Investor / Pogramme / Project type
-
Ministry of Education, Youth and Sports of the CR
- Large Infrastructures for Research, Development and Innovation
- MU Faculty or unit
-
Central European Institute of Technology
- prof. RNDr. Vladimír Sklenář, DrSc.
- Cooperating Organization
-
Institute of Biotechnology CAS
The Czech Infrastructure for Integrative Structural Biology (CIISB) is a distributed infrastructure of the core facilities and central laboratories of CEITEC (Central European Institute of Technology, Brno) and BIOCEV (Biotechnology and Biomedicine Centre, Vestec) providing expertise and access to technologies used for integrative approaches to structural analysis of biologically important cellular components and macromolecules - proteins, nucleic acids, and their complexes. CIISB, equipped with the cutting-edge technologies, will offer access to the equipment and expertise in the following fields: high-field NMR spectroscopy; high throughput crystallization of biological macromolecules; X-ray diffraction techniques; Bio-SAXS measurements; AFM imaging; characterization of proteins, nucleic acids and complexes by biophysical methods including microcalorimetry, dynamic light scattering, and surface plasmon resonance; high-end cryo-electron microscopy and tomography allowing studies of cellular structures, organelles, and biomacromolecular complexes by 3D imaging; protein expression in eukaryotic cells; high-end mass spectrometry characterization of biomacromolecules; determination of protein post-translational modifications; MS-based peptide mapping/sequencing; high throughput sequencing and proteomic services. CIISB infrastructure aims to provide a high quality open access services in provision of methods and techniques on a level of national and trans-national importance. CIISB is a part of the ESFRI project INSTRUCT-ERIC.
Sustainable Development Goals
Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.
Publications
Total number of publications: 327
2020
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OPTIMIZATION OF EXPRESSSION AND PURIFICATION OF FRAGMENT FROM TAU PROTEIN
Year: 2020, type: Conference abstract
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Phosphomimicking mutants: why to be careful
Year: 2020, type: Conference abstract
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Phosphorylation of multiple proteins involved in ciliogenesis by Tau Tubulin Kinase 2
Molecular Biology of the Cell, year: 2020, volume: 31, edition: 10, DOI
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Preparation of thorium dioxide nanofibers by electrospinning
Journal of Nuclear Materials, year: 2020, volume: 534, edition: JUN 2020, DOI
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RNF43 truncations trap CK1 to drive niche-independent self-renewal in cancer
EMBO Journal, year: 2020, volume: 39, edition: 18, DOI
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Structural Basis of Ca2+-Dependent Self-Processing Activity of Repeat-in-Toxin Proteins
MBIO, year: 2020, volume: 11, edition: 2, DOI
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Structure and mechanism of DNA delivery of a gene transfer agent
Nature Communications, year: 2020, volume: 11, edition: 1, DOI
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The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana
International Journal of Molecular Sciences, year: 2020, volume: 21, edition: 6, DOI
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The Effect of Synthesis Procedure on Hydrogen Peroxidase-Like Catalytic Activity of Iron Oxide Magnetic Particles
Applied Sciences-Basel, year: 2020, volume: 10, edition: 19, DOI
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The CH-pi Interaction in Protein-Carbohydrate Binding: Bioinformatics and In Vitro Quantification
Chemistry - A European Journal, year: 2020, volume: 26, edition: 47, DOI