Haloalkane Dehalogenases From Marine Organisms

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Authors

KUNKA Antonín DAMBORSKÝ Jiří PROKOP Zbyněk

Type Article in Periodical
Magazine / Source MARINE ENZYMES AND SPECIALIZED METABOLISM, PT B
MU Faculty or unit

Faculty of Science

Citation
Web https://loschmidt.chemi.muni.cz/peg/category/publications/#2018
Doi http://dx.doi.org/10.1016/bs.mie.2018.03.005
Keywords BIOCHEMICAL-CHARACTERIZATION; GAMMA-HEXACHLOROCYCLOHEXANE; BRADYRHIZOBIUM-JAPONICUM; DEGRADING BACTERIUM; EXPERIMENTAL-DESIGN; COLORIMETRIC ASSAY; PROTEIN STABILITY; ACCESS TUNNELS; STEADY-STATE; GEN. NOV.
Description Haloalkane dehalogenases degrade halogenated compounds to corresponding alcohols by a hydrolytic mechanism. These enzymes are being intensively investigated as model systems in experimental and in silico studies of enzyme mechanism and evolution, but also hold importance as useful biocatalysts for a number of biotechnological applications. Haloalkane dehalogenases originate from various organisms including bacteria (degraders, symbionts, or pathogens), eukaryotes, and archaea. Several members of this enzyme family have been found in marine organisms. The marine environment represents a good source of enzymes with novel properties, because of its diverse living conditions. A number of novel dehalogenases isolated from marine environments show interesting characteristics such as high activity, unusually broad substrate specificity, stability, or selectivity. In this chapter, the overview of haloalkane dehalogenases from marine organisms is presented and their characteristics are summarized together with an overview of the methods for their identification and biochemical characterization.
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