Computational Analysis of Protein Tunnels and Channels

Publikace nespadá pod Filozofickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři

BREZOVSKÝ Jan KOZLÍKOVÁ Barbora DAMBORSKÝ Jiří

Rok publikování 2017
Druh Kapitola v knize
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Popis Protein tunnels connecting the functional buried cavities with bulk solvent and protein channels, enabling the transport through biological membranes, represent the structural features that govern the exchange rates of ligands, ions, and water solvent. Tunnels and channels are present in a vast number of known proteins and provide control over their function. Modification of these structural features by protein engineering frequently provides proteins with improved properties. Here we present a detailed computational protocol employing the CAVER software that is applicable for: (1) the analysis of tunnels and channels in protein structures, and (2) the selection of hot-spot residues in tunnels or channels that can be mutagenized for improved activity, specificity, enantioselectivity, or stability
Související projekty: