Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability

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Publikace nespadá pod Filozofickou fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.
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KUNOVÁ Michaela MATULKA Kamil EISELLEOVÁ Lívia SALYKIN Anton KUBÍKOVÁ Iva KYRYLENKO Sergiy HAMPL Aleš DVOŘÁK Petr

Rok publikování 2013
Druh Článek v odborném periodiku
Časopis / Zdroj STEM CELLS Translational Medicine
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.5966/sctm.2012-0081
Obor Genetika a molekulární biologie
Klíčová slova Embryonic stem cells; Cell culture; Xenograft assay; Cell survival; Induced pluripotent stem cells
Popis The generation of human pluripotent stem cells (hPSCs) of sufficient quantity and quality remains a major challenge for biomedical application. Here we present an efficient feeder-free, high-density monolayer system in which hPSCs become SSEA-3-high and gradually more viable than their feeder-dependent counterparts without changes attributed to culture adaptation. As a consequence, monolayer hPSCs possess advantages over their counterparts in embryoid body development, teratoma formation, freezing as a single-cell suspension, and colony-forming efficiency. Importantly, this monolayer culture system is reversible, preserving the competence of hPSCs to gradually reacquire features of colony growth, if necessary. Therefore, the monolayer culture system is highly suitable for long-term, large-scale propagation of hPSCs, which is necessary in drug development and pluripotent stem cell-based therapies.
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