Cell differentiation along multiple pathways accompanied by changes in histone acetylation status

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Publikace nespadá pod Filozofickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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LEGARTOVÁ Soňa KOZUBEK Stanislav FRÁNEK Michal ZDRÁHAL Zbyněk LOCHMANOVÁ Gabriela MARTINET Nadine BÁRTOVÁ Eva

Rok publikování 2014
Druh Článek v odborném periodiku
Časopis / Zdroj BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://www.nrcresearchpress.com/doi/abs/10.1139/bcb-2013-0082?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed&#.VQAanuFFrs4
Doi http://dx.doi.org/10.1139/bcb-2013-0082
Obor Genetika a molekulární biologie
Klíčová slova NEWLY SYNTHESIZED HISTONES; EMBRYONIC STEM-CELLS; NUCLEAR ARRANGEMENT; REPLICATION FORK; H3K9 ACETYLATION; CHROMATIN; TRANSCRIPTION; H4; COMPLEX; SITES
Popis Post-translational modification of histones is fundamental to the regulation of basic nuclear processes and subsequent cellular events, including differentiation. In this study, we analyzed acetylated forms of histones H2A, H2B, and H4 during induced differentiation in mouse (mESCs) and human (hESCs) embryonic stem cells and during induced enterocytic differentiation of colon cancer cells in vitro. Endoderm-like differentiation of mESCs induced by retinoic acid and enterocytic differentiation induced by histone deacetylase inhibitor sodium butyrate were accompanied by increased mono-, di-, and tri-acetylation of histone H2B and a pronounced increase in di- and tri-acetylation of histone H4. In enterocytes, mono- acetylation of histone H2A also increased and tetra-acetylation of histone H4 appeared only after induction of this differentiation pathway. During differentiation of hESCs, we observed increased mono-acetylation and decreased tri-acetylation of H2B. Mono-, di-, and tri-acetylation of H4 were reduced, manifested by a significant increase in nonacetylated H4 histones. Levels of acetylated histones increased during induced differentiation in mESCs and during histone deacetylase (HDAC) inhibitor-induced enterocytic differentiation, whereas differentiation of human ESCs was associated with reduced acetylation of histones H2B and H4.
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