Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA

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Publikace nespadá pod Filozofickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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SIMONOVA Anna BÁLINTOVÁ Jarmila POHL Radek HAVRAN Luděk FOJTA Miroslav HOCEK Michal

Rok publikování 2014
Druh Článek v odborném periodiku
Časopis / Zdroj CHEMPLUSCHEM
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://onlinelibrary.wiley.com/doi/10.1002/cplu.201402194/full
Doi http://dx.doi.org/10.1002/cplu.201402194
Obor Elektrochemie
Klíčová slova DNA; electrochemistry; nucleotides; polymerase chain reactions; redox labeling
Popis 4-Hydroxy-3-methoxyphenyl (MOP) and 2,3-dihydrobenzofuran-5-yl (DHB) groups were tested as potential new oxidizable labels for electrochemical detection of DNA. The corresponding 5-aryl-cytosine and 7-aryl-7-deazaadenine 2-deoxyribonucleoside triphosphates (dNTPs) were prepared by the Suzuki coupling of halogenated dNTPs or by triphosphorylation of modified nucleosides, and were incorporated enzymatically into DNA by polymerase. In parallel, the electrochemical behavior of the labeled nucleosides, nucleotides, and DNA was studied to show that most of them give analytically useful signals of oxidation on the carbon electrode. Particularly, MOP appears to be a useful oxidizable DNA label since it offers independent detection of the modified DNA.
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