Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration

Varování

Publikace nespadá pod Filozofickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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ANYZ Jiri VYSLOUZILOVA Lenka VACULOVIČ Tomáš TVRDOŇOVÁ Michaela KANICKÝ Viktor HAASE Hajo HORAK Vratislav STEPANKOVA Olga HEGER Zbyněk ADAM Vojtěch

Rok publikování 2017
Druh Článek v odborném periodiku
Časopis / Zdroj Scientific Reports
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://www.nature.com/articles/srep40169
Doi http://dx.doi.org/10.1038/srep40169
Obor Analytická chemie, separace
Klíčová slova Bioanalytical chemistry; melanoma
Popis We describe a new procedure for the parallel mapping of selected metals in histologically characterized tissue samples. Mapping is achieved via image registration of digital data obtained from two neighbouring cryosections by scanning the first as a histological sample and subjecting the second to laser ablation inductively coupled plasma mass spectrometry. This computer supported procedure enables determination of the distribution and content of metals of interest directly in the chosen histological zones and represents a substantial improvement over the standard approach, which determines these values in tissue homogenates or whole tissue sections. The potential of the described procedure was demonstrated in a pilot study that analysed Zn and Cu levels in successive development stages of pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. We anticipate that the procedure could be useful for a complex understanding of the role that the spatial distribution of metals plays within tissues affected by pathological states including cancer.
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