p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice

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Publikace nespadá pod Filozofickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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BORA Pablo GAHUROVÁ Lenka MAŠEK Tomáš HAUSEROVÁ Andrea POTĚŠIL David JANSOVÁ Denisa SUSOR Andrej ZDRÁHAL Zbyněk AJDUK Anna POSPÍŠEK Martin BRUCE Alexander W.

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj Communications Biology
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
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Doi http://dx.doi.org/10.1038/s42003-021-02290-z
Klíčová slova ACTIVATED PROTEIN-KINASE; INNER CELL MASS; MAP KINASE; PREIMPLANTATION EMBRYO; MESSENGER-RNA; NA/K-ATPASE; 1A MYBBP1A; P38 MAPK; MOUSE; GROWTH
Popis Successful specification of the two mouse blastocyst inner cell mass (ICM) lineages (the primitive endoderm (PrE) and epiblast) is a prerequisite for continued development and requires active fibroblast growth factor 4 (FGF4) signaling. Previously, we identified a role for p38 mitogen-activated protein kinases (p38-MAPKs) during PrE differentiation, but the underlying mechanisms have remained unresolved. Here, we report an early blastocyst window of p38-MAPK activity that is required to regulate ribosome-related gene expression, rRNA precursor processing, polysome formation and protein translation. We show that p38-MAPK inhibition-induced PrE phenotypes can be partially rescued by activating the translational regulator mTOR. However, similar PrE phenotypes associated with extracellular signal-regulated kinase (ERK) pathway inhibition targeting active FGF4 signaling are not affected by mTOR activation. These data indicate a specific role for p38-MAPKs in providing a permissive translational environment during mouse blastocyst PrE differentiation that is distinct from classically reported FGF4-based mechanisms. Bora et al. show that an early blastocyst window of p38-MAPK activity regulates ribosome-related gene expression, rRNA precursor processing, polysome formation, and protein translation. This study suggests a distinct role of p38-MAPKs for providing a permissive translational environment during mouse blastocyst primitive endoderm differentiation.
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