In-gel digestion for mass spectrometric characterization of proteins and proteomes

Varování

Publikace nespadá pod Filozofickou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	HAVLIŠ Jan SHEVCHENKO Andrej MANN Mathias TOMAS Henrik OLSEN Jesper V.
Rok publikování	2008
Druh	Článek v odborném periodiku
Časopis / Zdroj	Nature Protocols
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
Obor	Genetika a molekulární biologie
Klíčová slova	ASSISTED-LASER-DESORPTION/IONIZATION; POLYACRYLAMIDE-GELS; NANOELECTROSPRAY; MODEL; SILAC; MS
Popis	In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.