Crucial factors governing the electrochemical impedance on protein-modified surfaces

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Publikace nespadá pod Filozofickou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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SOPOUŠEK Jakub VĚŽNÍK Jakub HOUSER Josef SKLÁDAL Petr LACINA Karel

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj Electrochimica Acta
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://www.sciencedirect.com/science/article/pii/S0013468621009063?via%3Dihub
Doi http://dx.doi.org/10.1016/j.electacta.2021.138616
Klíčová slova Electrochemical impedance spectroscopy; Polystyrene nanoparticles; Polyacrylic acid; Bovine serum albumin; Cationized bovine serum albumin; Ionic strength; Ionic charge
Popis Three different electrode surfaces - two nanoporous and one modified with polyelectrolyte - provided with positively and negatively charged model proteins were studied at different conditions to determine characteristics of electrochemical impedance spectroscopy in biosensing. The impedance response in the presence of [Fe(CN)(6)(4-/3-) was simultaneously influenced by two effects - ionic attraction/repulsion and steric hindrance of protein components. The ionic interactions dominated at low ionic strength and at low concentrations of redox probe. On the other hand, the steric hindrance of immobilized proteins affecting mass transfer towards the electrode gained impact gradually with the increasing ionic strength and with the concentration of the redox-active component. The simultaneous effect of these two variables can lead to confusing results which are often misinterpreted when evaluating results from impedimetric affinity biosensors. (C) 2021 Elsevier Ltd. All rights reserved.
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